Journal: International Journal of Nanomedicine

Article Title: Advances in Engineered Nanoparticles for the Treatment of Ischemic Stroke by Enhancing Angiogenesis

doi: 10.2147/IJN.S463333

Figure Lengend Snippet: Summary of Nanoparticles for Stroke Treatment by Angiogenesis

Article Snippet: Small extracellular vesicles secreted by mesenchymal stem cells (MSC) derived from human iPSC (iMSC-sEV) , Biomimetic nanoparticles , iMSC-sEV , Ischemic site , IV , Angiogenesis is a major obstacle to full recovery after a stroke, and iMSC-sEV, or small extracellular vesicles, released by MSCs generated from human iPSC, show tremendous promise in this regard. Treatment with iMSC-sEV prevented ischemia damage and increased angiogenesis, likely by preventing autophagy , After administration of iMSC-sEV, the number of CD31 + cells in the infarcted brain area further increased, which could promote angiogenesis , [ ] .

Techniques: Over Expression, Formulation, In Vitro, Activity Assay, Molecular Weight, Microinjection, Functional Assay, shRNA, Plasmid Preparation, Expressing, Adsorption, Permeability, Comparison, Cell Culture, Membrane, Disruption, Preserving, Liposomes, Derivative Assay, Generated, In Vivo, Control, Knock-Out, Injection

Journal: International Journal of Nanomedicine

Article Title: Advances in Engineered Nanoparticles for the Treatment of Ischemic Stroke by Enhancing Angiogenesis

doi: 10.2147/IJN.S463333

Figure Lengend Snippet: iMSC-sEV promote angiogenesis following stroke and increase HUVEC migration and tube formation after OGD. ( A – D ) Seven days after MCAO, angiogenesis was evaluated by immunofluorescence staining of CD31/EdU and CD34. Scale bar = 100 μm. * P < 0.05. Reproduced from Xia Y, Ling X, Hu G, et al. Small extracellular vesicles secreted by human iPSC-derived MSC enhance angiogenesis through inhibiting STAT3-dependent autophagy in ischemic stroke. Stem Cell Res Ther . 2020;11(1):313. Creative Commons.

Article Snippet: Small extracellular vesicles secreted by mesenchymal stem cells (MSC) derived from human iPSC (iMSC-sEV) , Biomimetic nanoparticles , iMSC-sEV , Ischemic site , IV , Angiogenesis is a major obstacle to full recovery after a stroke, and iMSC-sEV, or small extracellular vesicles, released by MSCs generated from human iPSC, show tremendous promise in this regard. Treatment with iMSC-sEV prevented ischemia damage and increased angiogenesis, likely by preventing autophagy , After administration of iMSC-sEV, the number of CD31 + cells in the infarcted brain area further increased, which could promote angiogenesis , [ ] .

Techniques: Migration, Immunofluorescence, Staining, Derivative Assay

Journal: International Journal of Nanomedicine

Article Title: Advances in Engineered Nanoparticles for the Treatment of Ischemic Stroke by Enhancing Angiogenesis

doi: 10.2147/IJN.S463333

Figure Lengend Snippet: Summary of Nanoparticles for Stroke Treatment by Angiogenesis

Article Snippet: Small extracellular vesicles (sEVs) prepared from mesenchymal stromal cell (MSC-sEVs) , Biomimetic nanoparticles , MSC-sEVs , Ischemic site , IV , Restorative stroke therapies can be achieved by using small extracellular vesicles (sEVs) derived from mesenchymal stromal cells (MSCs). The administration of MSC-derived sEVs in vivo resulted in improved neuronal plasticity and neurological recovery , sEVs obtained from hypoxic MSCs induce angiogenesis in vitro and promote microvascular network generation in vivo , [ ] .

Techniques: Over Expression, Formulation, In Vitro, Activity Assay, Molecular Weight, Microinjection, Functional Assay, shRNA, Plasmid Preparation, Expressing, Adsorption, Permeability, Comparison, Cell Culture, Membrane, Disruption, Preserving, Liposomes, Derivative Assay, Generated, In Vivo, Control, Knock-Out, Injection

Journal: International Journal of Nanomedicine

Article Title: Advances in Engineered Nanoparticles for the Treatment of Ischemic Stroke by Enhancing Angiogenesis

doi: 10.2147/IJN.S463333

Figure Lengend Snippet: iMSC-sEV promote angiogenesis following stroke and increase HUVEC migration and tube formation after OGD. ( A – D ) Seven days after MCAO, angiogenesis was evaluated by immunofluorescence staining of CD31/EdU and CD34. Scale bar = 100 μm. * P < 0.05. Reproduced from Xia Y, Ling X, Hu G, et al. Small extracellular vesicles secreted by human iPSC-derived MSC enhance angiogenesis through inhibiting STAT3-dependent autophagy in ischemic stroke. Stem Cell Res Ther . 2020;11(1):313. Creative Commons.

Article Snippet: Small extracellular vesicles (sEVs) prepared from mesenchymal stromal cell (MSC-sEVs) , Biomimetic nanoparticles , MSC-sEVs , Ischemic site , IV , Restorative stroke therapies can be achieved by using small extracellular vesicles (sEVs) derived from mesenchymal stromal cells (MSCs). The administration of MSC-derived sEVs in vivo resulted in improved neuronal plasticity and neurological recovery , sEVs obtained from hypoxic MSCs induce angiogenesis in vitro and promote microvascular network generation in vivo , [ ] .

Techniques: Migration, Immunofluorescence, Staining, Derivative Assay

Journal: International Journal of Nanomedicine

Article Title: Advances in Engineered Nanoparticles for the Treatment of Ischemic Stroke by Enhancing Angiogenesis

doi: 10.2147/IJN.S463333

Figure Lengend Snippet: When PMNs are absent in vivo, sEVs produced from hypoxic MSCs promote microvascular remodeling after ischemic stroke. ( A – C ) The microvascular network properties in the ischemic cerebral cortex were examined using 3D light sheet microscopy in mice that were subjected to 40 minutes of MCAO and then survived for 14 days. A, microvascular length density; B, branch point density; and C, mean branch length. ( D ) Magnified axial views of the striatum and cortex in an ischemic mouse brain showing the regions of interest for determining the properties of the microvascular network. Images of the ischemic cortex taken using maximum intensity projection ( E ) from each of the five groups. * p <0.05 compared with isotype/vehicle; # p <0.05 compared with isotype/sEV hypoxic . Scale bars=500 µm (in ( D )/100 µm (in ( E ). Reproduced from Gregorius J, Wang C, Stambouli O, et al. Small extracellular vesicles obtained from hypoxic mesenchymal stromal cells have unique characteristics that promote cerebral angiogenesis, brain remodeling and neurological recovery after focal cerebral ischemia in mice. Basic Res Cardiol . 2021;116(1):40. Creative Commons.

Article Snippet: Small extracellular vesicles (sEVs) prepared from mesenchymal stromal cell (MSC-sEVs) , Biomimetic nanoparticles , MSC-sEVs , Ischemic site , IV , Restorative stroke therapies can be achieved by using small extracellular vesicles (sEVs) derived from mesenchymal stromal cells (MSCs). The administration of MSC-derived sEVs in vivo resulted in improved neuronal plasticity and neurological recovery , sEVs obtained from hypoxic MSCs induce angiogenesis in vitro and promote microvascular network generation in vivo , [ ] .

Techniques: In Vivo, Produced, Microscopy